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− 60 mV − 120 mV pH Neutral form (%) K 0.5 (mmol/L)I max (nA)I max /K 0.5 (nA/mmol/L)K 0.5 (mmol/L)I max (nA)I max /K 0.5 (nA/mmol/L)Oocytes/batches (n /N ) PepT1a 6.5 98.4 0.24 ± 0.07 − 75.76 ± 6.04 316.37 0.45 ± 0.19 − 157.39 ± 21.48 350.81 9/3 7.6 83.0 6.92 ± 2.34 − 169.57 ± 43.75 24.51 3.61 ± 0.73 − 378.82 ± 53.08 105.02 14/3 PepT1b 6.5 98.4 0.13 ± 0.02 − 195.73 ± 8.89 1535.32 0.13 ± 0.02 − 396.24 ± 22.21 3032.16 7/1 7.6 83.0 2.22 ± 1.04 566.16 ± 212.44 254.54 1.01 ± 0.35 1142.30 ± 285.34 1129.67 7/1
Kinetic parameters (K 0.5 , I max , and I max /K 0.5 ) were calculated on Xenopus laevis oocytes voltage clamped at − 60 mV and at − 120 mV and perfused with Gly-Gln in sodium chloride buffer solutions at pH 6.5 and 7.6. Values are expressed as means ± SEM of n oocytes (each oocyte represents an independent observation). Kinetic parameters were calculated by least-square fit to the logistic equation (Fig. 2 ). I max /K 0.5 , transport efficiency
