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Table 1 Primer and probe sequences for the Met transporters and reference genes

From: Expression of proposed methionine transporters along the gastrointestinal tract of pigs and their regulation by dietary methionine sources

Gene Accession number Primer Sequence Probe sequence
B0AT1
(SLC6A19) 		XM_003359855.4 Fwd CTTCATCTTCACCCTGAACTC CCCCTGCTCATCATCGCCTTCTTCGAGATGT
Rev GATGTCGCTGTTGAACCTG
ATB0+
(SLC6A14) 		NM_001348402.1 Fwd CTGTGGCTTGGGGTGGTTTA CCAACTCCCAGGTGGGCCAT
Rev AACCAAGCAGCAACCCAAAG
rBAT
(SLC3A1) 		NM_001123042.1 Fwd CAATGCAGTGGGACAACAG TCCAAAAGACCCAGCCCAAATCAGCA
Rev GGCGTGAAGCAAACTTAATTC
ASCT2
(SLC1A5) 		XM_003127238.4 Fwd CGATTCGTTCCTGGATCTTG CTCCAACCTGGTGTCTGCAGCCTT
Rev TAGGACGTCGCGTATGAG
IMINO
(SLC6A20) 		XM_003358406.4 Fwd TCGTGTCCCTCATCAACAG ACCTCCATCTTTGCCAGTGTCGTCACCTT
Rev AGGAAGCCATCTTCAAGGTC
LAT4
(SLC43A2) 		XM_003358191.3 Fwd CAGATCCAGAAGATCACCAAC TGACCTGCCTCATTCCCAACCTGC
Rev TGAAGGAGAGAATCTGTAGGG
y+LAT1
(SLC7A7) 		NM_001110421.1 Fwd CTCTGCTGTTCAATGGTCTC GGCGCTGATCTACTTGTGCGTGGA
Rev ATAGAGCTGACCCACGATAG
LAT2
(SLC7A8) 		XM_003128550.5 Fwd ACTACCTCTTCTATGGCATCAC CGGACAGATAGTTCTTCGCTGGAAGAAGCCTAA
Rev GCAAGTAGATGATGGGGAACAG
SNAT2
(SLC38A2) 		XM_003126626.5 Fwd TTCATTCTTCCATCTGCCTTC GTTAAGTGGCATAGGGGTGATGACCGGA
Rev GGGCATTGTGTACCCAATC
ACTB XM_021086047.1 Fwd GACATCAAGGAGAAGCTGTG CTGGACTTCGAGCAGGAGATGGCC
Rev CGTTGCCGATGGTGATG
GAPDH XM_021091114.1 Fwd CAAGAAGGTGGTGAAGCAG TGAGGACCAGGTTGTGTCCTGTGACTTCAA
Rev GCATCAAAAGTGGAAGAGTG
YWHAZ XM_005662949.2 Fwd AAGAGTCATACAAAGACAGCAC ATCGGATACCCAAGGAGATGAAGCTGAA
Rev ATTTTCCCCTCCTTCTCCTG
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Genes & Nutrition

ISSN: 1865-3499

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